Investigation of molecular variability in some Aegilops species using Start Codon Targeted Polymorphism (SCoT) and CAAT-Box Derived Polymorphism (CBDP) markers

Among wild relatives of wheat, Aegilops species are ideal genetic resources for the discovery new characteristics such as resistance to environmental stresses and even grain quality wheat improvement. Hence, knowledge population structure diversity this germplasm is very important their conservation further utilization. In present study, 80 accessions including Ae. tauschii, cylindrica triuncialis were investigated using SCoT CBDP markers. Eight SCOT twelve primers amplified a total 84 94 fragments with mean 10.50 7.83 per primer, respectively. Resolving power (Rp) varied between 6.04 11.65, 13.08 28.02, polymorphic information content (PIC) from 0.40 0.49 0.35 0.48, The results analysis molecular variance (AMOVA) indicated that highest proportion referred species. high values all informativeness parameter (except resolving power) than across tested accessions. However, higher parameters primers. As result, maximum number effective alleles (Ne), Nei?s gene (H) Shannon?s index (I) detected in markers, Cluster based on those system grouped into three main clusters. grouping pattern observed by more clear phylogenetic relationship among some species, so PCoA?s confirmed pattern. conclusion, it was displayed good efficiency depicting polymorphism accessions, however, markers provided evaluated use determining estimating other plant recommended.